Aivia Image Analysis Software from Leica Microsystems

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ItemAivia Image Analysis Software
CompanyLeica Microsystems
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Leica Microsystems

10 Parkway North Blvd., Suite 300

Deerfield, Illinois 60015

United States

Phone: 800 248 0123

Company Profile

Website: www.leica-microsystems.com

(1) Mitochondria isolated from lipid droplets of white adipose tissue reveal functional differences based on lipid droplet sizeLife Sci AllianceDecember 6, 2023Alexandra J Brownstein, Michaela Veliova, Rebeca Acin-Perez, Frankie Villalobos, Anton Petcherski, Alberto Tombolato, Marc Liesa, Orian S Shirihaianalysis Fat layers of large and small LDs stained with BD493 were measured by fluorescence detection using AIVIA image analysis software (version 10.5.0; Leica Microsystems). The LDs were detected and segmented by BD493 fluorescence using

(2) Medium Depth Influences O2 Availability and Metabolism in Human RPE CulturesInvestigative Opthalmology & Visual ScienceNovember 3, 2023Daniel T. Hass, Qitao Zhang, Gillian A. Autterson, Richard A. Bryan, James B. Hurley, Jason M. L. Millerobtained using the Leica STELLARIS 8 FALCON Confocal Microscope, and image analysis was performed with Aivia 11 (Leica, North Deerfield, IL, USA). To quantify lipid droplets (LDs), a machine learning pixel classifier

(3) Mobility gene expression differences among wild-type, Mmp20 null and Mmp20 over-expresser mice plus visualization of 3D mouse ameloblast directional movementScientific ReportsNovember 1, 2023Masashi Shin, Aya Matsushima, Jun-ichi Nagao, Yoshihiko Tanaka, Hidemitsu Harada, Koji Okabe, John D. Bartlettatmosphere of 5% CO2 within a stage top incubator (Tokai Hit). The time-lapse images were processed using Aivia software (Leica Microsystems). Cells were identified using pixel classifier and individual cell tracking

(4) Activation of CB2R by synthetic CB2R agonist, PM289, improves brain endothelial barrier properties, decreases inflammatory response and enhances endothelial repairNeuroImmune Pharmacology and TherapeuticsOctober 16, 2023Trent A. Bullock, Kalpani N. Udeni Galpayage Dona, Jonathan F. Hale, Paula Morales, Nadine Jagerovic, Allison M. Andrews, Servio H. Ramirezconfocal microscope (Nikon Inc, Melville, NY). Image analysis for particle counting was performed with the AIVIA Imaging software (Leica Microsystems, Wetzlar, Germany). Isolation of brain microvasculature Following

(5) A Next-Generation 3D Tissue-Engineered Model of the Human Brain Microvasculature to Study the Blood-Brain BarrierBioengineering (Basel, Switzerland)July 8, 2023Galpayage Dona KNU, Ramirez SH, Andrews AM.fluorescence microscope at T = 0 min and 10 min at 10× magnification. Permeability was quantified using Aivia imaging software (Leica) and determined by calculating the percent permeability from the fluorescent

(6) The mechanisms to dispose of misfolded proteins in the endoplasmic reticulum of adipocytesNature communicationsMay 30, 2023Wu SA, Shen C, Wei X, Zhang X, Wang S, Chen X, Torres M, Lu Y, Lin LL, Wang HH, et al.(Thermo Fisher Scientific) and Imaris x64 9.5.1 (Oxford Instruments). Segmentation was performed using AIVIA 10 (Leica): manual segmentation was performed to determine the boundaries of the CERF in 30 images of

(7) Actin filaments accumulated in the nucleus and remain in the vicinity of condensing chromosomes during prophase in zebrafish early embryoBiology OpenMay 15, 2023Haruka Oda, Yuko Sato, Shigehiro A. Kawashima, Yusuke Fujiwara, Máté Pálfy, Edlyn Wu, Nadine L. Vastenhouw, Motomu Kanai, Hiroshi Kimura(version 2.1.0). For the cell cycle length measurement in Fig. S6B, a microscopy image analysis software AIVIA (version 11.0.1, Leica) was used to track individual nuclei in 3D. Drawing line graphs, box plots, and

(8) Meshed neuronal mitochondrial networks empowered by AI-powered classifiers and immersive VR reconstructionFrontiers in NeuroscienceFebruary 2, 2023Shu-Jiao Li, Hui Liu, Fei-Fei Wu, Da-Yun Feng, Shuai Zhang, Jie Zheng, Lu Wang, Fei Tian, Yan-Ling Yang, Ya-Yun Wangmitochondrial networks of cerebellar Purkinje cells were composed of all continuous 2D images of Z-stack by Aivia (version 10.5.1, LeiCa, Germany) (Harada et al., 2012; Hintiryan et al., 2021). Aivia uses artificial

(9) Caspase-9 driven murine model of selective cell apoptosis and efferocytosisCell Death & DiseaseJanuary 24, 2023Lena Batoon, Amy J. Koh, Rahasudha Kannan, Laurie K. McCauley, Hernan Rocawithin the selected threshold) was recorded. Cleaved caspase-3+ signals in the spleen were analyzed with AIVIA (Leica Microsystem) using 20X tiled images of the entire section. The pixel-based iterative training

(10) Aging compromises human islet beta cell function and identity by decreasing transcription factor activity and inducing ER stressScience advancesOctober 7, 2022Shrestha S, Erikson G, Lyon J, Spigelman AF, Bautista A, Manning Fox JE, Dos Santos C, Shokhirev M, Cartailler JP, Hetzer MW, et al.size and density of LAMP1-positive granules in the beta cell cytoplasm, we created pixel classifiers [Aivia software (v10.5), Leica Microsystems] for INS and LAMP1 channels. This generated binary segmentation

(11) A Statistical Porosity Characterization Approach of Carbon-Fiber-Reinforced Polymer Material Using Optical Microscopy and Neural NetworkMaterials (Basel)September 21, 2022Sara Eliasson, Mathilda Karlsson Hagnell, Per Wennhage, Zuheir Barsoumcommon and continuously developed. In biomedicine, the work is growing, and microscope software such as Aivia (by Leica Microsystems, Inc) implements Artificial Intelligence (AI) solutions to analyze images. Ilastic

(12) Identification of embryonic RNA granules that act as sites of mRNA translation after changing their physical propertiesiScienceJune 17, 2022Sato K, Sakai M, Ishii A, Maehata K, Takada Y, Yasuda K, Kotani T.observed under an N-SIM super-resolution microscope (Nikon). The volume of signals was measured by AIVIA software (Leica). Collection, fixation and section in situ hybridization of mouse embryos were performed

(13) A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissuesNature MethodsMay 11, 2022Adam K. Glaser, Kevin W. Bishop, Lindsey A. Barner, Etsuo A. Susaki, Shimpei I. Kubota, Gan Gao, Robert B. Serafin, Pooja Balaram, Emily Turschak, Philip R. Nicovich, et al.colonies Quantitative morphological analysis of metastatic lumen and cancer cells (Fig. 3) were completed in Aivia 9.8.1 (Leica Microsystems, Bellevue, WA). First, Aivia’s GPU accelerated machine learning-based Pixel

(14) Caspase-2 regulates S-phase cell cycle events to protect from DNA damage accumulation independent of apoptosisOncogeneJanuary 5, 2022Ashley G. Boice, Karla E. Lopez, Raj K. Pandita, Melissa J. Parsons, Chloe I. Charendoff, Vijay Charaka, Alexandre F. Carisey, Tej K. Pandita, Lisa Bouchier-Hayesprobability threshold 0.4–0.7 depending on the set). Raw data and CellPose masks were imported into Aivia 9.8 (Leica Microsystems) and the mean fluorescence intensity was measured in each of the Cyan and Venus

(15) Deletion of ABCB10 in beta-cells protects from high-fat diet induced insulin resistanceMolecular MetabolismJanuary 1, 2022Michael Shum, Mayuko Segawa, Raffi Gharakhanian, Ana Viñuela, Matthew Wortham, Siyouneh Baghdasarian, Dane M. Wolf, Samuel B. Sereda, Laura Nocito, Linsey Stiles, et al.TPLC core, from which total pancreas and islet areas (DAB-stained area) were quantified. The software Aivia v9.1 was used to quantify the percentage of Ki67-positive beta-cells, the latter identified by immunofluorescence

(16) Imaging in focus: An introduction to denoising bioimages in the era of deep learningThe International Journal of Biochemistry & Cell BiologyNovember 1, 2021Romain F. Laine, Guillaume Jacquemet, Alexander Krulle.g, Nikon’s Denoise.AI and Zeiss’ Apeer, or from DL-focused image analysis platforms such as Leica’s Aivia. Additionally, denoising is also commonly used in the field of natural images such as photography or

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